DNA extraction

Today, we are going to demostrate to extract the DNA from vegetables cells. To do it, we have to need of some materials:
-glaves
-termomether
-ice
-glass’ container
-scales
-sodium cloride
-liquid soap
-water
-alcool
-ripe fruit
PROCEDIMENT
1. First we have to prepare the intractis solution: liquid soap 10% and sodium cloride 3% and water and we gently stirring without to prevede bubbles.
2. We peel the fruit and we mash it in the „mortaio”.
3. We put mush inside a becker (50 ml of mush)
4. We have to add extraction’s solution (other 50 ml)
5. Then we put the becker at ’’bagnomaria” to 60° C and we stir the mush to conform the temperature.
6. After,exactly 5 minute we remove the becker, we putting it to cool in the water for stop the lysis reaction. Stirring to conform the temparature.
7. Then two minutes and half we have to removethe becker by cold water and we filter it.
8. We take 3 ml of the filtrate and we put it inside a glass’ cylinder , and slowly we add 6 ml of cold alcool, keeping thecylinder inclined to 45°, to do deposit the alcool above of filtrate.
9. We leave to rest for 5 minutes.
10. The DNA, which extracted from the cells, precipitates and appears as a kind of gel.
11. Now, we can take it, putting it on a clean slide, coloring it with a Metilene’s blue and observing it at microscope.